Figure 1.

Physical maps of the centromere-proximal regions of the right arms of chromosomes III and IV. Contig numbering follows that of the Broad Institute (http://www.broad.mit.edu/annotation/fungi/aspergillus/). Distances between genes or to the end of the available genomic sequence are given. Coordinates following the gene name indicate the coding region with that of the first base of the initiation codon appearing first. Contigs 1.161 and 1.162 were joined by alignment with contig 1.198. To join contigs 1.162 and 1.163, genomic DNA was PCR amplified using oligonucleotides annealing at a distance of 170 nt from the end of 1.162 and the beginning of 1.163. Sequencing of the PCR fragment revealed database errors at the ends of both contigs (a consequence of shotgun cloning) and, after deletion of a total of 36 incorrect nt, a 34-nt addition was necessary to join the two contigs. The chromosome III contigs thus joined in total 429,190 bp, but only 429,190 bp of the 627,835 bp of contig 1.129 from chromosome IV are indicated.