TABLE 1.

Mutant sequence changes determined in this work

Gene	Database notation	Allele	Nucleotide change	Amino acid change	Mutant protein
phenA	NAa	phenA2	A100Gd	IVS1g	1–18 + 15
phenA4	A100G	IVS1g	1–18 + 15
phenA5	859insTT	F268fsh	1–267 + 19
phenA7	G29A	G10D	G10D
phenA8	G421A	W121i	1–120
halA	AN8830.2	halA24	1566insATCAACTCGTCC	insINSS	1–503 + INSS + 504–524
cbxA/carC	AN8793.2b	cbxA17	A684G	H156R	H156R
carC9	T483C	L89P	L89P
suaB	Gln-tRNAc	suaB111	G145,904Ae	—	—
hisA	AF159463	hisA10	T973C	L296S	L296S
AN7430.2
gdhB	AY223544	gdhB1	ΔT1014-T1023	M318fs	1–317 + 12
AN7451.2
meaA	AY049706	meaA8	C855Tf	Q231i	1–230
	AN7463.2

^aGene not annotated by the database. Predicted product is prephenate dehydratase; cDNA was sequenced.

^bPredicted product is cytochrome B-560 subunit of succinate dehydrogenase; cDNA was sequenced.

^cPredicted glutamine tRNA, anticodon CUG. Start is at 145,869, end at 145,954. Contig 1.161. Amber suppressor. G-to-A change in the anticodon sequence in suaB111.

^dNucleotide coordinates are based on the coding region in genomic DNA.

^eContig 1.161 coordinate; change in Figure 1 coordinate is G283,287A.

^fOur nucleotide sequence change corrects that given by Monahan et al. (2002).

^gIntron lariat sequence was altered; splicing defect is likely. The predicted protein would terminate after translation of the (mutant) intron and the first eight nucleotides of exon 2.

^hfs, frameshift.

ⁱStop codon.