AtHD1 is a single-copy gene and is expressed in
Arabidopsis. (A) A simplified restriction
map of AtHD1. Arrows indicate the primers used to
amplify a 1.5-kb reverse transcription–PCR fragment. The fragments
used as DNA probes are indicated below the diagram. (B)
Autoradiogram showing a DNA blot containing genomic DNA from Columbia
(C) and Landsberg (L), which was hybridized with the 3′ region (≈500
bp) of AtHD1 (A). Ba,
BamHI; Dr, DraI; EI,
EcoRI; EV, EcoRV. (C) An
RNA blot was hybridized with a full-length cDNA fragment as a probe
(A). At, A. thaliana, Ca,
Cardaminopsis arenosa; As, A. suecica.