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. 2000 Dec 26;98(1):200–205. doi: 10.1073/pnas.011347998

Figure 6.

Figure 6

AtHD1 reduction and histone H4 hyperacetylation in CASH 141. Crude protein extracts (25 μg) or histone fractions (2 μg) were loaded onto a SDS/PAGE gel and then immunoblotted onto Immobilon-P (Millipore) or Hybond-ECL (Amersham Pharmacia). The membrane was probed with antibodies against the N terminus of AtHD1 and antibodies specific for nonacetylated (H4) or tetraacetylated histone H4 (AcH4). (A) AtHD1 in CASH141 was reduced to 10% (lane 4) of the level in a control plant (lane 3). Protein loading control is shown in an 8% SDS/PAGE stained with Coomassie blue (lanes 1 and 2). (B) Histone H4 was hyperacetylated ≈10-fold in the CASH141 (lane 4) compared with the wild-type plant (lane 3). An equal amount of nonacetylated histone H4 was detected (lanes 1 and 2).