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Comparison of kinetic constants determined by using CLiPS, fluorescent protein FRET substrates, or synthetic peptides
| Substrate | Sequence |
kcat/KM, μM−1min−1 |
||
|---|---|---|---|---|
| CLiPS | CyPet-YPet | Synthetic | ||
| Linker | GGSGGS | — | 0.19 ± 0.03 | — |
| Canonical | DDDDK | 1.3 ± 0.2 | 57 ± 6 | 7.1 ± 0.7 |
| EP4.1 | VDYRFL | 9.7 ± 0.2 | 520 ± 80 | — |
| EP4.2 | MHGERM | 12.4 ± 1.4 | 480 ± 30 | — |
| EP4.3 | SGDRMW | 18.0 ± 9.3 | 1,020 ± 120 | 36 ± 15 |
| EP4.6 | SGERMM | 14.7 ± 3.3 | 710 ± 40 | — |
