TABLE 3.
Deficiencies enhance GMR-dRetWT, GMR-dRetMEN2A, and GMR-dRetMEN2B
| Deficiency | Breakpoints | GMR-dRetWT | GMR-dRetMEN2A | GMR-dRetMEN2B | Candidate gene, alleles tested | Interaction |
|---|---|---|---|---|---|---|
| Df(1)C128 | 7D1;7D5 | SE(100) | SE(100) | SE(100) | myospheroida: mys1 | N |
| Df(1)RA2 | 7D18;8A4 | SE(100) | ME(100) | ME(100) | Neuroglian: Nrg14, Nrg17 | WE |
| Df(1)KA14 | 7F1;8C6 | WE(100) | WE(100) | ME(100) | Neuroglian: Nrg14, Nrg17 | WE |
| Df(1)lz-90b24 | 8B5;8D9 | SE(100) | WE(45) | SE(100) | Moesin: MoeG0404, MoeG0415 | WE |
| Df(1)sd72b | 13F1;14B1 | WE(78) | WE(26) | ME(100) | vap(RasGAP) | — |
| Df(1)B25 | 15D3;16A6 | WE(67) | WE(62) | WE(90) | — | |
| In(2LR)DTD16LDTD42R | 23C;23E6 | WE(68) | SE(100) | ME(84) | Chd1b | — |
| Df(2L)cact-255rv64 | 35F;36D | SE(88) | ME(92) | SE(100) | cadN2: cadN2BG02611a | WE |
| Df(2L)H20 | 36A8;36F1 | ME(100) | SE(90) | ME(100) | cadN2: cadN2BG02611a | WE |
| Df(2L)TW137 | 36C2;37B10 | SE(89) | SE(100) | ME(100) | cadN2: cadN2BG02611a | WE |
| Df(2L)TW50 | 36E4;38A7 | WE(86) | SE(100) | ME(91) | Paxillin, PaxEY02020 | WE |
| Df(2R)vg-C | 49B2;49E2 | SE(85) | SE(98) | SE(96) | Sin3A: Sin3A08269, Sin3Aex4 | WE |
| Df(3R)T-32 | 86C1;87B5 | WE(100) | WE (58) | WE(93) | dCsk: dCskj1D8 | WE |
| Df(3R)M-Kxl | 86E2;87C7 | WE(88) | ME(79) | WE(79) | dCsk: dCskj1D8 | WE |
| Df(3R)e-N19 | 93B2;94A8 | WE(78) | SE(96) | WE | Rab11c: Rab1193Bi, Rab11j2D1 | N |
| cortactind | — | |||||
| RhoGAP93Be:RhoGAP93BEY06358 | N | |||||
| Df(3R)e-R1 | 93B6;93D4 | WE(54) | SE(100) | ME(100) | Rab11c: Rab1193Bi, Rab11j2D1 | N |
| cortactind | — | |||||
| RhoGAP93Be:RhoGAP93BEY07163 | N |
Each deficiency is listed by name. The cytological breakpoints for each are derived from the FlyBase online database and the Bloomington Stock Center. Genetic interactions are indicated according to strength of phenotype: M, moderate; S, strong; W, weak; and type of interaction: S, suppressor; E, enhancer; N, no interaction. Therefore, SS is a strong suppressor. Parentheses indicate penetrance of the interaction calculated as a percentage. —, indicates that there were no mutants in the candidate gene available for testing.
mys encodes a PS integrin β-subunit, which are proteins that regulate focal adhesions and Src signaling (Brown 1994).
Chd1 encodes a chromatin-remodeling protein very similar to dMi-2 (Stokes et al. 1996).
Rab11 encodes a small GTPase that regulates endosomal trafficking of transmembrane receptors (Cullis et al. 2002).
cortactin encodes a known substrate for Src kinases (Wu et al. 1991; Okamura and Resh 1995).
RhoGap93B is an ortholog of RhoGAP, which itself is a regulator of RetMEN2 activity (Chiariello et al. 1998).