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. 2005 Nov;171(3):1057–1081. doi: 10.1534/genetics.104.038018

TABLE 5.

Genetic interactions between GMR-dRetMEN2B P-element modifiers and GMR-dRetWT, GMR-dRetMEN2A, and dEGFRElp

Gene/allele GMR-dRetMEN2B GMR-dRetMEN2A GMR-dRetWT MEN2B specific dEGFRElp
msnj1E2 ME(88) WE(18) WE(50) No WE
dCskj1D8 WE(76) N WE(52) No N
hhrJ413 WE(84) WE(92) ME(100) No SE
crbj1B5 WE(93) WE(69) WE(100) No N
l(3)j2D5 ME(95) WE(76) WE(82) No WS
drkk02401 SS(95) N N? Noa WS
spis3547 WS(86) WS(65) No MEb
pxk08316 WS(73) N WS(54) No WE
Sk09538 WS(67) WS(14) N Noa MEb
kismetk10237 WS(80) WS(71) N No ND
drkk13809 WS(100) N WS(23) No N
ebik16213 SS(85) WS(65) WS(18) No N
Ras85D06677 WS(76) MS(100) No ND
l(3)06803 WS(95) MS(91) No WE
l(3)06906 WS(100) MS(100) No N
l(3)j5B6 WE(97) ME(100) No N
l(3)j4B9 WE(95) WE(68) No N
neurj6B12 WE(94) WE(47) No N
Pp1-87Bj6E7 WE(85) WE(29) No WE
scribj7B3 WE(98) WE(45) No ME
Sin3A08269 WE(86) WE(100) WE(77) No N
drk10626 SS(100) WS(24) MS(100) No N
l(3)S005504 ME(91) WE(30) WE(70) No N
l(3)S001405 WE(95) N WE(58) No WE
l(3)S003704 WE(94) N ND WE
l(3)S000718 WE(96) N WE(47) No N
l(3)S009515 WE(93) N WE(62) No N
l(3)S000710 WE(82) WE(26) No N
l(3)S012805 WE(100) N WE(97) No N
l(3)S016805 WE(88) WE(12) WE(96) No N
l(3)S023708 ME(94) WE(15) N No N
l(3)S023549 WE(82) WE(50) No N
l(3)S024833 WE(96) WE(33) No N
l(3)S023901 WE(100) WS(39) WE(79) No N
l(3)S022231 WE(91) N WE(80) No N
l(3)S024503 WE(92) N ND N
l(3)S026238 WE(100) N WE(65) No N
l(3)S026421 WE(94) N WE(90) No N
l(3)S024329 WE(88) WE(71) WE(52) No N
l(3)S049706 ME(85) N WE(82) No ND
l(3)S054513 WE(79) N ND N
l(3)S046604 WE(96) WE(52) ME(95) No N
l(3)S049902 WE(93) N WE(35) No WE
DlS049520 WS(85) WS(100) No N
bonS048706 WE(93) WE(50) WE(81) No WS
l(3)S047526 ME(100) N SE(100) No WE
l(3)S066607 WE(96) WE(22) WE(96) No N
l(3)S057101 WE(95) N WE(59) No WE
l(3)S068808 WE(93) WE(32) WE(84) No N
l(3)S083407 WE(90) WE(24) No N
l(3)S063512 WE(81) WE(15) WE(75) No ME
l(3)S056113 WE(88) N WE(50) No N
l(3)S090101 WE(100) WE(38) WE(81) No N
l(3)S090114 WE(79) N WE(76) No N
l(3)S141715 WE(84) N ND N
l(3)S136603 WE(97) N WE(90) No N
l(3)S092708 WE(94) N WE(83) No N
l(3)S003003 WE(82) N WE(100) No WE
l(3)S050116 WE(97) WE(49) WE(46) No N
l(3)S142909 WE(84) N WE(85) No N
l(3)S147412 WE(91) WE(39) WE(85) No N
l(3)S145911 WE(91) N WE(69) No N
l(3)S146006 WS(100) MS(100) WS(52) No N
l(3)S135703 WE(86) N WE(81) No N
l(3)S133117 WE(85) WE(45) WE(81) No N
l(3)S130910 WE(85) WS(30) WE(40) No WE

Each GMR-dRetMEN2B modifier was crossed to GMR-dRetWT and/or GMR-dRetMEN2A constructs to determine the specificity of dRetMEN2 genetic interactions. Not all P-element lines could be tested since some lines died and were no longer available from the stock centers: these are indicated as ND (not determined).

a

Other alleles of Star and drk interact with GMR-RetMEN2A (Table 6). This is probably due to the hypomorphic nature of the alleles isolated in the screen.

b

Some positive regulators of the Ras pathway such as spitz actually dominantly enhanced dEGFRElp, demonstrating that dEGFRElp does not show simple linear interactions with Ras-ERK kinase pathway components. This suggests that dEGFRElp does not constitute a simple gain-of-function model for RTK signaling; this may reflect negative feedback loops that regulate dEGFR signaling (Spencer and Cagan 2003).