TABLE 6.
Genetic interactions between GMR-dRet isoforms and additional alleles of candidate P-element modifiers
| Gene | Gene function | Allele or deficiency | GMR-dRetMEN2B | GMR-dRetMEN2A | GMR-dRetWT | Comments | Decision |
|---|---|---|---|---|---|---|---|
| misshapen | Ste20 serine/threonine kinase | msnj1E2 | ME | WE(18) | WE(50) | Modifier | |
| msn03349 | ME(61) | ||||||
| dCsk | C-terminal Src kinase | dCskj1D8 | WE | N | WE(52) | dCskj1D8 is a strong hypomorph; deficiencies pinpoint dCsk region. | Modifier |
| Df(3R)M-Kxl | WE(79) | ME(79) | WE(88) | ||||
| Df(3R)T-32 | WE(93) | WE(58) | WE(100) | ||||
| Df(3R)T-61 | WE (80) | ||||||
| hedgehog | Secreted ligand in Hedgehog pathway | hhrJ413 | WE | WE(92) | ME(100) | hhAC is null; hh2 and hh21 are hypomorphs. | Modifier |
| hhneo56 | WS(32) | ||||||
| hhAC | WE(24) | ||||||
| hh2 | WE(15) | WE(73) | |||||
| hh21 | N | ||||||
| drk | Ortholog of Grb2 | drkk02401 | SS | N | N | drk10626 is the strongest allele; none are true nulls. | Modifier |
| drkk13809 | WS | N | WS(23) | ||||
| drk10626 | SS | WS(24) | MS(100) | ||||
| spitz | dEGFR ligand | spis3547 | WS | WS(65) | Modifier | ||
| spi01068 | WS(46) | ||||||
| spi1 | WS(56) | WS(40) | |||||
| plexus | Nuclear factor involved in Ras pathway | pxk08316 | WS | N | WS(54) | pxk08134 is a weak hypomorph. | Unclear |
| pxk08134 | N | ||||||
| Df(2R)X58-12 | SS(100) | SS(100) | MS(80) | ||||
| Star | Protein required to process and activate spitz | Sk09538 | WS | WS(14) | N | Modifier | |
| SIIN | WS(42) | WS(25) | |||||
| Sk09530 | WS(63) | N | |||||
| kismet | Chromodomain protein involved in Ras pathway | l(2)k10237 | WS | WS(71) | N | Noncomplementation with known kismet alleles confirms that l(2)k10237 and l(2)k16510 are kismet mutations. | Modifier |
| l(2)k16510 | WS(36) | ||||||
| kisk13416 | WS(79) | N | |||||
| Df(2L)net-PMF | WS(100) | WS(86) | WS(83) | ||||
| ebi | Nuclear protein involved in Ras pathway | ebik16213 | SS | WS(65) | WS(18) | Modifier | |
| Df(2L)al | SS(100) | MS(100) | SS(100) | ||||
| Ras85D | Ras ortholog | Ras85D06677 | WS | MS(100) | Ras85DΔc40b is a null. | Modifier | |
| Ras85DΔc40b | WS | ||||||
| Pp1-87B | Phosphatase | Ppi-87Bj6E7 | WE | WE(29) | Unclear | ||
| Pp1-87B1 | WE(10) | N | WE(24) | ||||
| Pp1-87B2 | WE(29) | WS(47) | |||||
| Df(3R)ry615 | WE(17) | ||||||
| Sin3A | Transcriptional corepressor | Sin3A08269 | WE | WE(100) | WE(77) | Sin3Aex4 is a null. | Modifier |
| Sin3AHW52 | ME(98) | ||||||
| Sin3Ak07401 | ME(100) | ||||||
| Sin3Ak08919 | SE(82) | ME(85) | |||||
| Sin3Aex4 | ME(93) | WE(52) | ME(97) | ||||
| Df(2R)vg-C | SE(96) | SE(98) | SE(85) | ||||
| Mi-2 | Chromodomain protein and ATP- dependent helicase | l(3)S005504 | ME | WE(30) | WE(70) | Noncomplementation with Mi-2j3D4 confirms l(3)S005504, l(3)S047526, and l(3)S147412 as dMi-2 alleles. | Modifier |
| l(3)S047526 | ME | N | SE(100) | ||||
| l(3)S147412 | WE | WE(39) | WE(85) | ||||
| Mi-2j3D4 | ME(52) | ||||||
| Delta | Transmembrane ligand for the Notch receptor | DlS049520 | WS | WS(100) | Modifier | ||
| Dl9P | WS(78) | MS(88) | |||||
| crumbs | Transmembrane cell adhesion receptor | crbj1B5 | WE | WE(69) | WE(100) | crb8F105 and crb2 are nulls. | False |
| crb8F105 | WS(18) | ||||||
| crb2 | WE(41) | N | |||||
| l(3)07207 | WS(22) | WS(63) | WE(23) | ||||
| Df(3R)crbF89-4 | N | ||||||
| scribble | Cell polarity protein | scribj7B3 | WE | WE(45) | scrib1 and scrib2 are nulls. | False | |
| scrib1 | N | WS(59) | |||||
| scrib2 | N | WS(67) | |||||
| string | Cell cycle regulator | l(3)S024503 | WE | Complementation tests performed in Salzberget al. (1997) | False | ||
| l(3)S022406 | N | ||||||
| l(3)S043922 | N | ||||||
| l(3)S073013 | N | ||||||
| l(3)S089605 | N | ||||||
| stg01235 | N | ||||||
| Df(3R)3450 | N | ||||||
| neuralized | Ubiquitin ligase | neurj6B12 | WE | WE(23) | neur1 is a strong hypomorph; neur11 is a null. | False | |
| neur11 | N | N | WE(29) | ||||
| neur1 | N | ||||||
| neur3 | N | ||||||
| neurA101 | WS(24) | WS(79) | |||||
| neurneo37 | N | ||||||
| cyclin A | Cyclin A ortholog | l(3)S054513 | WE | Complementation tests performed by Salzberget al. (1997) | False | ||
| l(3)S003302 | N | ||||||
| l(3)S004024 | N | ||||||
| l(3)S004623 | N | ||||||
| l(3)S010407 | N | ||||||
| l(3)S024534 | N | ||||||
| l(3)S052106 | N | ||||||
| l(3)S144905 | N | ||||||
| CycA03946 | N |
Allelic strength is generally noted in the FlyBase online database based on published literature. Candidates were called “modifier” if more than an additional allele or matching deficiencies showed reproducible and highly penetrant genetic interactions with one or more form of GMR-dRet. Candidates were called “false” if null alleles failed to interact. Many additional alleles showed low penetrance interactions, making the results “unclear.”