Cut protein levels, but not Sv protein levels, are reduced in GMR∷Z eye discs. (A and C) Wild-type eye discs. (B and D) GMR∷Z/GMR∷Z eye discs. Late third instar eye imaginal discs were immunostained for Sv (A and B) and Cut (C and D). Note the loss of Cut staining in the GMR∷Z disc (D), as compared to the wild-type disc (C). In D, the arrow refers to the peripodial cells, which are part of the membrane that surrounds the disc. Some of these cells show staining with the anti-Cut antibody, but the staining is not in the disc itself.