Figure 9.

Z physically associates with Pax5. HeLa cells were transfected with expression plasmids for Z alone (lanes 2, 4, and 6), Pax5 alone (lanes 1 and 5) or Z plus Pax5 (lanes 3 and 7). Anti-Pax5 antibody was used to co-immunoprecipitate the Pax5/Z complex (lanes 1–3). An anti-Z antibody (bottom) and an anti-Pax5 antibody (top) were used for immunoblotting. The anti-Z antibody immunoblots revealed the co-immunoprecipitated Pax5/Z complex (lane 3), as well as the levels of Z protein present in the cell extracts used (lanes 6 and 7). The anti-Pax5 antibody immunoblots revealed the levels of Pax5 protein immunoprecipitated with the anti-Pax5 antibody (lanes 1–3), as well as the levels of Pax5 proteins present in the cell extracts used (lanes 5 and 7).