Figure 2.

A genomic map of the wdl locus (A) shows the relative locations of microsatellite markers. TGCE detected an abnormality in exon 8 of the Car8 gene (B). PCR amplification of Car8 exon 8 generated a smaller band in wdl/wdl mice, a larger band in +/+ mice, and two bands in +/wdl mice (C). Comparison of PCR products from eight mouse strains and wdl/wdl mice (D) revealed that the deletion does not exist in any normal mouse strains (E). DNA sequence analysis revealed a 19-bp deletion in Car8 exon 8 from wdl/wdl mice (F).