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. 2005 Nov;171(3):885–899. doi: 10.1534/genetics.105.044719

Figure 4.

Figure 4.

Gene dosage-dependent effects of ASF1 on telomeric silencing. TPE assays: (A) Suppression of the telomeric silencing defect in cac1Δ cells by low-level overexpression of ASF1. Wild-type (PKY090) and cac1Δ (PKY638) strains isogenic for URA3-VIIL were transformed with empty vector (pRS415; ARS-CEN-LEU2 plasmid) or a plasmid containing the ASF1 gene (pPK196; ARS-CEN-LEU2-ASF1). Transformants were plated onto synthetic media lacking leucine (−Leu) with and without 5′-FOA. (B) 2μ-level ASF1 overexpression disrupts silencing in cac1Δ cells. Wild-type and cac1Δ strains were transformed with empty vector (YEP35l; 2μ-LEU2 plasmid) or a 2μ-plasmid containing the ASF1 gene. (pPK197; 2μ-LEU2-ASF1). Transformants were plated onto synthetic media lacking leucine (−Leu) with and without 5′-FOA.