TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pRS414 | ARS-CEN-TRP1 | Sikorski and Hieter (1989) |
| pRS415 | ARS-CEN-LEU2 | Sikorski and Hieter (1989) |
| pPK196 | ARS-CEN-LEU2-ASF1 | This study |
| YEP35l | 2μ-LEU2 | Lee et al. (2003) |
| pPK197 | 2μ-LEU2-ASF1 | This study |
| pMS383 | ARS-CEN-TRP1-GAL-HHT1 | Mitch Smith (University of Virginia) |
| pPK128 | 2μ-LEU2-HHT1-HHF1 HTA1-HTB1 | Kaufman et al. (1998) |
| pRS423 | 2μ-HIS3 | Sikorski and Hieter (1989) |
| pMH151-7 | 2μ-HIS3-CRT1 | Huang et al. (1998) |
| pBAD54 | 2μ-TRP1 + GAP promoter | Desany et al. (1998) |
| pBAD70 | 2μ-TRP1 + GAP-RNR1 | Desany et al. (1998) |
| pBAD79 | 2μ-TRP1 + GAP-RNR3 | Desany et al. (1998) |
| pRAD53 | ARS-CEN-LEU2-RAD53 | Lee et al. (2003); Schwartz et al. (2003) |
| prad53fha1 | ARS-CEN-LEU2-rad53 R70A N107A | Lee et al. (2003); Schwartz et al. (2003) |
| prad53fha2 | ARS-CEN-LEU2-rad53 N655A V666A S657A | Lee et al. (2003); Schwartz et al. (2003) |
| prad53kd | ARS-CEN-LEU2-rad53 K227A D339A | Lee et al. (2003); Schwartz et al. (2003) |