Figure 2.

Reactivity of Retromer Antibodies and Localization of Retromer Proteins in Subcellular Fractions of Arabidopsis and Tobacco BY-2 Cells.

P13 is the membrane pellet after a 13,000g centrifugation for 20 min; P100 is the pellet resulting from a subsequent 1-h centrifugation at 100,000g with the P13 supernatant; S100 is the resulting supernatant after 100,000g centrifugation. Protein gel blots were probed with VPS35 or VPS26 antibodies from Arabidopsis or yeast (Vps35p) recombinant proteins. Forty micrograms of protein was loaded in each lane.