Figure 5.

Sedimentation Characteristics of Retromer Binding Membranes in Sucrose Gradients.

(A) Arabidopsis P100 membranes were separated on an isopycnic 15 to 55% (w/w) linear sucrose gradient, and the precipitated fractions were protein gel blotted with antibodies against the ER membrane marker calnexin, the Golgi marker RGP (reverse glycosylated protein), the plasma membrane marker PM-ATPase, the prevacuolar markers VSR_At-1 and PEP12 (SYP21), and VPS35 antibodies.

(B) As for (A) but with tobacco BY-2 P100 membranes.