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Immunogold Localization of Retromer to MVBs in Tobacco BY-2 Cells.
Thin sections were prepared from high-pressure frozen/freeze-substituted specimens and immunostained with antibodies against the three proteins of the large retromer subunit (VPS35, VPS29, and VPS26). Labeling tends to be found at the surface of the multivesicular body. Arrowheads point to internal vesicles that are seldom labeled. Bar = 200 nm.
