Figure 2.

GI Is Required for the Rhythmicity of CAB at 12 and 27°C.

Transgenic seedlings carrying the CAB:LUC reporter gene were entrained under 12L:12D cycles for 7 d, after which the seedlings were transferred to 12, 17, or 27°C and constant red and blue light. Luminescence was monitored in the wild-type Ws (closed squares) and gi-11 (open triangles). For both the wild type and gi-11, expression was monitored in at least three independently transformed lines.

(A) Plots represent the normalized average expression of 8 to 27 single seedlings of one representative transformed line. Error bars indicate se.

(B) Summary of the mathematical analysis of the expression rhythms of the seedlings, represented by plots of period estimates plotted against the respective relative amplitude errors (Rel. Amp. Error) for wild-type Ws and gi-11. At 27°C, wild-type n = 31, gi-11 n = 26; at 17°C, wild-type n = 116, gi-11 n = 51; at 12°C, wild-type n = 52, gi-11 n = 43.