Figure 4.

GI mRNA Expression Is Weakly Temperature-Regulated.

Wild-type seedlings were grown at 22°C in 12L:12D conditions for 7 d, and the seedlings were then transferred to constant light and 12°C (open squares), 17°C (closed squares), or 27°C (closed triangles). They were harvested after 72 h and every 4 h thereafter for the next 24 h. Total RNA was assayed by real-time quantitative PCR, and the accumulation of GI was measured relative to an internal UBQ control. Error bars indicate se. Each point represents the average of three biological repeats, with each biological repeat made up of three technical repeats.