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. 2006 May;18(5):1121–1133. doi: 10.1105/tpc.105.039834

Figure 2.

Figure 2.

Expression of amiRNAs and Cleavage of Predicted Targets.

(A) RNA gel blot analysis of amiRNA overexpressers using a mixture of probes for all amiRNAs. The outermost lanes contain two standards. miR156a and miR172a overexpressers were included as controls. Lane 1, amiR-mads-2 (MIR319a backbone); lane 2, amiR-mads-1 (MIR172a backbone); lane 3, amiR-trichome (MIR319a backbone); lane 4, amiR-lfy-2 (MIR319a backbone); lane 5, amiR-lfy-2 (MIR172a backbone); lane 6, amiR-lfy-1 (MIR172a backbone); lane 7, amiR-yabby-2 (MIR319a backbone). M, size marker; nt, nucleotides.

(B) Mapping of target cleavage products by rapid amplification of cDNA ends using PCR. Fraction of sequenced clones with particular 5′ end indicated on top. In the case of LEAFY (LFY), only one clone had a 5′ end at the expected position, opposite nucleotides 10 to 11 of the intended amiRNA. The 5′ end of most clones was offset by two nucleotides, suggesting that most of the amiRNAs were offset as well. The sequence predicted from the aberrant processing is indicated in gray.