Figure 5.

Inducible and Tissue-Specific Expression of amiRNAs.

Uninduced or wild-type controls are shown at the left.

(A) Ethanol-induced ubiquitous expression of amiR-white-1 3 and 5 d after induction. After 3 d, young leaves are all yellow; after 5 d, the youngest leaves are green again.

(B) Ethanol-induced ubiquitous expression of amiR-trichome (right) 3 d after induction. Clustered trichomes appear as white covering of youngest leaves (arrowhead).

(C) Inflorescences of plants expressing amiR-white-1 from the AP1 promoter (middle) are pale yellow. Strong lines expressing amiR-mads-2 from the AP1 promoter (right) resemble ap1 cal double mutants.

(D) Expression of amiR-lfy-1 from the LFY promoter (1) results in flowers resembling lfy mutants. amiR-mads-2 expressed from AG regulatory elements in the center of the flower (2) produces organ transformations in the central two whorls. Outer whorls remained unaffected. An opposite phenotype was seen after expression of amiR-mads-2 from the AP1 promoter (3; weaker line), which didn't affect inner whorls but resulted in secondary flowers, resembling ap1 mutants.

(E) Epidermal expression of amiR-white-1 from the MERISTEM LAYER1 (ML1) promoter resulted in pale plants.