Figure 7.

d-Glucose Has a Direct Effect on THF1 Steady State Level.

(A) Green fluorescence in root cells expressing a 35S-driven THF1-GFP fusion protein disappeared 30 min after being treated with 6% d-glucose, but not l-glucose

(B) THF1-GFP degradation at the indicated applied glucose levels (%) for 30 min was confirmed by immunoblotting probed with anti-GFP antiserum. Degradation did not occur with l-glucose treatment and was inhibited by the general protease inhibitor MG132. Not shown are nontransformed roots, which did not display fluorescence under these excitation and digital capture conditions.