Figure 7.

Loss-of-Function Mutants for the WRKY Genes.

(A) Diagram of WRKY18, WRKY40, and WRKY60 and their T-DNA insertion or transposon tagging mutants.

(B) RNA gel blot analysis of wrky18-1, wrky40-1, and wrky60-1 mutants. Wild-type and mutant plants were infiltrated with PstDC3000 (OD₆₀₀ = 0.01). Two hours after the inoculation, the inoculated leaves were harvested and total RNA was isolated. After separation on the gel and blotting to a nylon membrane, the blot was probed with gene-specific DNA fragments. The RNA gel blotting was repeated at least three times with independently isolated RNA with similar results.