Figure 1.

Histone deacetylase (HDAC)1 protein depletion occurs after treatment of cells with tumour necrosis factor-α (TNF-α). Breast carcinoma (ZR-75-1) and immortalized normal breast (76N-E6) were treated with 20 ng/ml recombinant human TNF-α for the indicated durations and then collected. Western blots of SDS–polyacrylamide gel electrophoresis-resolved cytoplasmic and nuclear extract proteins from these cells probed with different class I HDAC and control protein antibodies and visualized by chemiluminescence are shown. PARP, poly(ADP-ribose) polymerase.