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. 2005 Dec 23;7(3):291–296. doi: 10.1038/sj.embor.7400613

Figure 3.

Figure 3

Histone deacetylase 1 protein depletion requires IKK2. (A) ZR-75-1 cells were treated with 5 ng/ml interleukin-1β (IL-1β), 10 μg/ml lipopolysaccharide (LPS) or 50 ng/ml TNF-related apoptosis-inducing ligand (TRAIL) for the indicated durations and then collected. Western blots of nuclear extract proteins probed with histone deacetylase (HDAC)1 and poly(ADP-ribose) polymerase (PARP) antibodies are shown. (B) Wild-type (wt) mouse embryo fibroblasts (MEFs) and their complementary cells containing homozygous deletions of IKK2−/− or RelA−/− were treated with 20 ng/ml tumour necrosis factor-α (TNF-α) for the indicated durations. Western blots of nuclear extract proteins probed with HDAC1 and PARP antibodies are shown. (C) Normal Panc-28 pancreatic cancer cells or those constitutively expressing the IκBα super-repressor were treated with 20 ng/ml TNF-α for the durations indicated, then collected. Western blots of cytoplasmic and nuclear extract proteins probed for HDAC1, p65, IκBα, PARP and paxillin are shown.