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. 2006 Jan 20;7(3):283–290. doi: 10.1038/sj.embor.7400614

Figure 1.

Figure 1

FLRT proteins interact physically with each other. (A) Human embryonic kidney 293T cells were co-transfected with the indicated tagged constructs and 48 h later were collected for a co-immunoprecipitation (co-IP)–western blot analysis. (B) Co-IP of 293T cells transfected with V5-tagged Xenopus FLRT3 and the indicated Renilla luciferase (RL)-tagged constructs. RL activity after co-IP was normalized against total RL activity of the lysates. (C) 293T cells were co-transfected with human FLRT2–RL fusion and the indicated enhanced yellow fluorescent protein (EYFP)-tagged constructs and analysed by bioluminescence resonance energy transfer (BRET) assay. Data are represented as BRET ratio, which is defined as E530/E470(sample)−E530/E470(hFLRT2–RL only). WCL, whole-cell lysate; xBMPRI, Xenopus BMP type I receptor; xFGFR1, Xenopus fibroblast growth factor receptor 1; xFz8, Xenopus Frizzled 8.