Skip to main content
. 2001 Jan 2;98(1):206–211. doi: 10.1073/pnas.98.1.206

Figure 3.

Figure 3

14-fold mpxPCR. (a) A 5-plex PCR was performed with primers 1–5 (Table 1); (b) 4-plex PCR with primers 2, 3, 5, and 8 (Table 2); (c) 5-plex PCR with primers 1, 4, 6, 7, and 9 (Table 2): (d) 14-plex PCR with primers 1–5 (Table 1) plus primers 1–9 (Table 2). Primers 2, 3, and 5 (Table 2) were normal types. Other conditions were as in Fig. 2. The 450-bp fragment (aldolase B gene; primer 4 in Table 2) appeared as a double peak, as it also did in a uniplex PCR (data not shown). The 1-kb-long fragment (product generated by the primer 5 in Table 1) is beyond the displayed window. The fragment's lengths (bp) are indicated above the peaks. The 200-bp-long IL2 amplicon is marked by an arrow.