Figure 4.

Different DNA polymerases display different specificity in mpxPCR. 4-plex PCR used primers 1, 2, 3, and 4 in Table 1. Thermostable DNA polymerase (2.5 units) or a mixture of two DNA polymerases (2.5 units) was used in PCR. Other conditions were as in Fig. 2. PD, primer-dimers.