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Genotyping of CF human DNA samples. (A) Uniplex reactions with primers targeting normal type (1), ΔF508 CFTR-homozygous (2), and ΔF508 CFTR-heterozygous (3) DNA. The PCR products were analyzed on a 2% agarose gel. (B) Genotyping of the same DNA samples in a 4-plex PCR. In this reaction, CFTR normal type (N) and CFTR mutant (M) primers (primers 10 and 11, respectively, in Table 2) were used in a mixture with primers 1, 2, and 4 (Table 1) (see Materials and Methods for the details).
