Table 1.

PCR primers targeted at anonymous sequences in human chromosome 7 used in PS mpxPCR

Primer (5′–3′)	Direction, length (nt), GC content	Product length (bp)
		Expected	Obtained
1. AATGCCTGCCATGTATAAGCTACCCGGTC	Reverse, 29, 15/29	165	165
2. GTCCCGTCCCCATCCTCACAAGCTGTCGC	Reverse, 29, 19/29	285	290
3. AGTGCCCATGCCCGTGAGACCTGGAGAAG	Direct, 29, 18/29	507	510
4. CCGGAGGAAATTGGAGTAGACTCGGAAGAG	Direct, 30, 16/30	212	210
5. GCAGCCCCAAGCACCAAGCTGAGCAAACAG	Direct, 30, 18/30	970	≈1,000
			150