Table 2.
Quantification of mitotic defects after 48 h CDK11 RNAi in GFP–CDK11P58 and GFP–CDK11P110 cells
| Cell line | Treatment: | Mitotic index | Prophase* | Prometaphase* | Metaphase* | Anaphase+telophase* | Cytokinesis* | Monopolar+short spindles* |
|---|---|---|---|---|---|---|---|---|
| GFP–CDK1p58 | Control | 10.3±0.6 | 1.5±0.5 | 5.1±1.2 | 13.2±2.1 | 1.5±0.6 | 78.7±3.6 | 0 |
| CDK11 RNAi | 11.3±1.4 | 1.5±0.6 | 4.4±0.8 | 13.8±1.6 | 1.5±0.6 | 78.9±2.7 | 0 | |
| GFP–CDKp110 | Control | 6.9±0.3 | 6.2±2.1 | 13.8±0.9 | 21.1±3.4 | 5.9±1.0 | 53.0±1.2 | 0 |
| CDK11 RNAi | 21.4±3.1 | 3.3±0.7 | 11.4±2.9 | 4.4±0.4 | 4.9±1.9 | 43.5±3.0 | 34.7±2.0 | |
| CDK11, cyclin-dependent kinase 11; RNAi, RNA interference. | ||||||||
| * Values are a percentage of the total mitosis in each category ±s.d. | ||||||||