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. 2006 Jan 27;7(4):425–430. doi: 10.1038/sj.embor.7400624

Figure 1.

Figure 1

Depletion of Cdc6 in human cells results in G1/S block and cell death. (A) HeLa cells transfected for 72 h with mock (water) or 120 nM luciferase (Luc) or Cdc6 endonuclease-prepared small interfering RNA (esiRNA) were subjected to immunoblot analysis with anti-Cdc6, anti-Orc2, anti-Cdt1, anti-MCM7 or anti-α-tubulin. (B) HeLa cells transfected as in (A) were fixed and analysed by fluorescence-activated cell sorting. (C) HeLa and HCT116 cells transfected as in (A) were fixed and stained with 4,6-diamidino-2-phenylindole. The percentages of catastrophic cells were scored (>500 cells) using a fluorescence microscope.