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. 2006 Jan 27;7(4):425–430. doi: 10.1038/sj.embor.7400624

Figure 2.

Figure 2

Depletion of Cdc6 in early G1 phase blocks G1 progression, and depletion of Cdc6 in early S phase results in mitotic cell death. (Left) HeLa cells were synchronized at G2/M by a thymidine–nocodazole block and released into early G1 phase by the addition of fresh medium for 3 h. The early-G1-phase cells were transfected with mock or luciferase (Luc) or Cdc6 endonuclease-prepared small interfering RNA (esiRNA), and grown for another 9 h before fluorescence-activated cell sorting (FACS) analysis or immunoblotting using anti-Cdc6 antibodies. (Right) HeLa cells were synchronized at G1/S by a double-thymidine block and released into early S phase by the addition of fresh medium for 1 h. The early-S-phase cells were transfected with mock or Luc or Cdc6 esiRNA, and grown for another 19 h before FACS analysis or immunoblotting using anti-Cdc6 antibodies.