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Depletion of Cdc6 in S phase neither activates nor functionally abolishes Chk1 surveillance. HeLa cells in early S phase were transfected with luciferase (Luc) or Cdc6 endonuclease-prepared small interfering RNA (esiRNA). After 5 h, cells were treated for further 3 h with or without 5 μg/ml aphidicolin. Cellular proteins were immunoblotted with anti-pS317-Chk1, anti-Chk1, anti-Cdc6 or anti-PLCγ1 (control) antibodies.
