Figure 2.

Kinetics of RNA pol II occupancy at the promoter or distal exon of DHFR and GAPDH genes (A) after UV irradiation (10 J/m²). Soluble chromatin was prepared from WT and CS8PV cells at indicated times after UV treatment and subjected to ChIP assay using the indicated antibodies. Real-time PCR using specific primers was performed to test the relative enrichment for either the proximal promoter (initiation) or the distal regions (elongation) of the gene as compared to the unirradiated samples. Kinetics of RNA pol II occupancy either at the promoter (B) or at a distal region (C) of DHFR gene after UV irradiation, in WT and CSB deficient cells. Kinetics of TBP (D), TFIIB (E), CSB (F) and acetylated H4 (G) at the promoter of DHFR gene. RNA pol II occupancy after UV irradiation, at the promoter of DHFR, either in CSB (CS1AN) or in CS1AN cells (CS1AN+CSB/WT) transfected with WT CSB gene (H). RNA pol II occupancy at the promoter of DHFR gene in WT cells previously transfected with a pool of oligonucleotides either of control (siCTRL) or silencing CSB gene (siCSB) (I). RNA pol II occupancy either at the promoter (J) or at a distal region (K) of GAPDH gene in WT and CSB cells. The results are expressed as fold enrichment relative to the untreated cells. Data are from three independent experiments. The values shown are means±s.d.