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. 2006 Apr 6;25(9):1934–1944. doi: 10.1038/sj.emboj.7601079

Figure 7.

Figure 7

siRNA-mediated reduction of PABP affects translation. (A) HeLa cells were transfected with the indicated HA-Paip2 plasmids and siRNAs as in Figure 3C. At 24 h following the second transfection, cells were transfected with in vitro transcribed capped Fluc mRNA. Cells were harvested 6 h later, and extracts were subjected to Luciferase assay. Data are expressed as the percentage of Fluc activity in cells transfected with control siRNA and WT HA-tagged Paip2 (set at 100%). Error bars denote the standard deviation from three independent experiments. (B) Western blot analysis of the cell extracts from (A) was performed with the indicated antibodies. (C) HeLa cells were transfected with the indicated siRNAs as in Figure 6B. At 24 h following the second siRNA transfection, cells were transfected with Fluc mRNA and then analyzed as in (A). Data are expressed as the percentage of Fluc activity in cells transfected with control siRNA (set at 100%). Error bars denote the standard deviation from three independent experiments. (D) Western blot analysis of the cell extracts from (C) was performed with the indicated antibodies.