Table 1.

Primers and DGGE screening conditions

Primers*	cDNA Sequence	Fragment size (bp)	Gradient %**	Running T (°C)
Forward 5'-GTAATAACTCTGGAACTGACAA-3'	1998–2308	350	40–70	50
Reverse 5'-GC-GGACTATCTGAACTTTGCACTA-3'
Forward 5'-CAGGTATCAAGATGGACAGC-3'	2197–2461	304	40–70	50
Reverse 5'-GC-TGAACCTTGGAATTTTGAGTAG-3'

* (GC) = 5'-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG-3' ** 100% denaturant = 7M urea and 40% (v/v) formamide in TAE buffer