Figure 1.

Thalidomide blocks angiogenesis. Fourth day incubated chicken eggs were broken, entire egg contents were transferred into a sterile petri dish. In vascular bed, heart and blood capillary are visible on 0 hour panel (left). Next, the vascular bed was incubated with 100 μg/ml thalidomide disc, placed on the vascular bed for 12 hours. It is evident from the representative images that 100 μg/ml thalidomide retarded the growth of the blood capillaries at the edges of vascular bed. A 70% loss of blood vessels (inset on top of the panels) was estimated by counting the number of red pixels using an analytical module of Adobe Photoshop ver. 6.0.