Figure 9.
Thalidomide attenuates DEAN induced tube formation in ECs. ECs were seeded on collagen plated 12 well plates with 60% cell density, to form monolayers after 24 hours of incubation at 37°C/5% CO2. Total number of DEAN (10 μmol) induced tubes were counted after 12 hours of incubation with thalidomide (25, 50, and 75 μgm/ml). * P < 0.05 compared to only DEAN treated cells.