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Chromosome terminus location in live cells. (A) RTP-YFP and cell outline. (B) 181°∷lacO, LacI-CFP, and cell outline. (C) Merged image A and B. (D) Percent of cells belonging to each type of cell pattern in double-labeled cells (283 total cells). The remaining 2.7% of cells counted did not contain signal from both fusions. Orange circles, RTP-YFP; blue squares, LacI-CFP bound to the chromosome terminus at 181°∷lacO. The data are cumulative from two separate experiments.
