Table 2.
The rtp enhancement of anucleate cell production is recA dependent
| Relevant genotype | Strain* | Anucleates | Total cells | Percentage of anucleates† |
|---|---|---|---|---|
| Wild type | AG174 | 8 | 8,693 | 0.09 (0.06) |
| recA260 | IRN444 | 66 | 6,218 | 1.06 (0.25) |
| Δrtp recA260 | IRN440 | 46 | 6,910 | 0.67 (0.19) |
| ΔspoIIIE recA260 | IRN441 | 39 | 3,622 | 1.08 (0.34) |
| Δrtp ΔspoIIIE recA260 | IRN439 | 59 | 6,040 | 0.98 (0.25) |
| ΔripX recA260 | IRN442 | 63 | 6,654 | 0.95 (0.23) |
| Δrtp ΔripX recA260 | IRN443 | 44 | 4,126 | 1.07 (0.31) |
*
Strains were handled as described in Materials and Methods, except that the ethanol fixation time was decreased to ≈10 s to preserve cellular structure in strains carrying the recA260 mutation. Identical results were obtained with two different isolates of the ripX recA strains (IRN442 and IRN443), and results shown are cumulative.
†
All data are derived from at least two experiments. The 95% confidence interval is indicated in parentheses.