Figure 4.

The genomic loci of eya (A), Optix (B), shf (C), and VhaPPA1–1 (D). Ey binding sites shown to bind Ey by EMSAs are shown as grey ovals, predicted binding sites that did not bind Ey as open ovals. Reporter fragments responsive to Ey are shown as gray bars. In the transcribed regions, untranslated sequence is shown as white boxes and the coding region is shown in gray. The shf1 and Optix2/3 reporters are expressed in the third instar eye disc and are induced by ectopic ey; the eya3 reporter is only induced by ectopic ey. In A, a previously described 322-bp eye enhancer is indicated by a black bar.