Figure 6.

In vivo reporter activity of Ey binding sites from shf and Optix. α-GFP is shown in green; α-Dac is shown in magenta in all panels. (A) A construct containing an Ey binding site in shf drives GFP in the eye disc anterior to the morphogenetic furrow. This construct also drives expression in the antennal disc, near the anterior/posterior (A/P) compartment boundary of the wing disc (B), and in the leg disc (not shown). A merge of A and B is shown in C. In a dpp-GAL4/UAS-ey background, the shf1 reporter is strongly induced throughout the dpp expression domain along the A/P boundary of the wing disc. (D) A construct containing the Optix2 and Optix3 binding sites (Optix2/3) expresses strongly throughout the eye disc and in no other imaginal tissues examined (a wing disc is shown in E). Optix2/3 is also strongly induced in the wing disc throughout the dpp expression domain of dpp-GAL4/UAS-ey animals. A merge of D and E is shown in F. Arrowheads in A and B indicate reporter activity in a wild-type background. Arrows in C and F indicate ectopic induction.