Figure 5.

Schematic diagram of designing primers used for allelic-specific expression analysis of LRK6. Two small allelic indels in the 5′ and 3′ noncoding regions of LRK6 were used for designing the allelic-specific primers. Lightly shaded rectangles, coding regions; darkly shaded rectangles, non-coding regions; black lines, allelic indels. Primer1, primer specific for Nipponbare (or Dongxiang wild rice, DW); Primer2, primer specific for 93–11 (or Guichao2, GC2). RT-PCR was performed using one allelic-specific primer and another primer derived from the coding region.