Figure 1.

Dose-dependent induction by IFN of Ifi47 (A), Tap1 (B) and Mx1 (C) expression. Real-time PCR was performed on cDNAs prepared from MEFs (WT, NFκB-KO and p50-KO) treated with IFNβ at varying concentrations for 5 hrs. Gene expression was normalized to actin expression in each sample. Data are shown as fold-induction relative to untreated fibroblasts, and are mean values ± the SEM (n=3).