Northern blot analysis of mitochondrial C1-THF synthase transcripts in adult human tissues. A multiple human tissue RNA blot was hybridized with 32P-labeled probes to the 5′ end (panel A) or 3′ end (panel B) of human mitochondrial C1-THF synthase cDNA. In panel C, the membrane was hybridized with a probe from the 3′ end of the human cytoplasmic C1-THF synthase cDNA. The lanes on each membrane contain RNA from (left to right) brain, placenta, skeletal muscle, heart, kidney, pancreas, liver, lung, spleen, and thymus. The schematic diagram below shows the relative locations of the probes used for panels A and B on the 3.6 and 1.1 kb transcripts.