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. 1978 May;34(5):947–954.

Specificity of murine delayed-type hypersensitivity to conjugates of large or small haptens on protein carriers bearing lipid groups.

H Snippe, L Johannesen, J K Inman, B Merchant
PMCID: PMC1457205  PMID: 77841

Abstract

Delayed-type hypersensitivity (DH) in the mouse was provoked with different hapten-carrier complexes mixed with the cationic, surface-active lipid, dimethyl dioctadecyl ammonium bromide (DDA). DH was measured as footpad swelling. Conjugates of bovine serum albumin (BSA) with the small haptens dinitrophenyl (DNP), 'arsonate' (ARS) and 'sulphonate' (SULPH) served to generate strong DH reactions towards the homologous antigen. Insertion of a tripeptide spacer between the hapten and carrier resulted in lower DH reactivity. Optimal dosages and optimal time intervals between sensitization and DH elicitation were determined for the enlarged hapten-carrier complexes. Cyclophosphamide (CY) treatment, before priming with complexes mixed with DDA, caused a 5-6 day delay in the expression of DH but failed to evoke enhanced DH for any of the antigens tested. A broad array of cross reactions between small and enlarged hapten-carrier complexes showed a relative lack of specificity in these DH responses. The results are compared with others reported in the literature and are explained mainly by the effects of electrostatically bound lipid groups of DDA in the sensitizing conjugates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dailey M. O., Hunter R. L. The role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity. J Immunol. 1974 Apr;112(4):1526–1534. [PubMed] [Google Scholar]
  2. Inman J. K., Merchant B., Claflin L., Tacey S. E. Coupling of large haptens to proteins and cell surfaces: preparation of stable, optimally sensitized erythrocytes for hapten-specific, hemolytic plaque assays. Immunochemistry. 1973 Mar;10(3):165–174. doi: 10.1016/0019-2791(73)90005-0. [DOI] [PubMed] [Google Scholar]
  3. Inman J. K., Merchant B., Tacey S. E. Synthesis of large haptenic compounds having a common functional group that permits covalent linkage to proteins, cell surfaces, and adsorbents. Immunochemistry. 1973 Mar;10(3):153–163. doi: 10.1016/0019-2791(73)90004-9. [DOI] [PubMed] [Google Scholar]
  4. Inman J. K. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975 Feb;114(2 Pt 1):704–709. [PubMed] [Google Scholar]
  5. Janeway C. A., Cohen B. E., Ben-Sasson S. Z., Paul W. E. The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues. J Exp Med. 1975 Jan 1;141(1):42–55. doi: 10.1084/jem.141.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Janeway C. A., Maurer P. H., Dailey M. O., Inman J. K. The specificity of cellular immune responses II. The structure of antigenic determinants leading to T-lymphocyte stimulation. J Exp Med. 1976 Dec 1;144(6):1621–1640. doi: 10.1084/jem.144.6.1621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Janeway C. A., Paul W. E. The specificity of cellular immune responses in guinea pigs. III. The precision of antigen recognition by T lymphocytes. J Exp Med. 1976 Dec 1;144(6):1641–1656. doi: 10.1084/jem.144.6.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kerckhaert J. A., Van den Berg G. J., Willers J. M. Influence of cyclophosphamide on the delayed hypersensitivity of the mouse. Ann Immunol (Paris) 1974 Mar-Apr;125(3):415–426. [PubMed] [Google Scholar]
  9. Merchant B., Inman J. K. Hapten-specific hemolytic plaque assays usually fail to detect most of the diversity in the anti-hapten response. J Exp Med. 1977 Feb 1;145(2):372–389. doi: 10.1084/jem.145.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Paul W. E. Functional specificity of antigen-binding receptors of lymphocytes. Transplant Rev. 1970;5:130–166. doi: 10.1111/j.1600-065x.1970.tb00359.x. [DOI] [PubMed] [Google Scholar]
  11. Snippe H., Belder M., Willers J. M. Dimethyl diotadecyl ammonium bromide as adjuvant for delayed hypersensitivity in mice. Immunology. 1977 Dec;33(6):931–936. [PMC free article] [PubMed] [Google Scholar]
  12. Snippe H., Willems P. J., Graven W. G., Kamp E. Delayed hypersensitivity in the mouse induced by hapten-carrier complexes. Immunology. 1975 May;28(5):897–907. [PMC free article] [PubMed] [Google Scholar]
  13. Stashenko P. P., Schlossman S. F. Antigen recognition: the specificity of an isolated T lymphocyte population. J Immunol. 1977 Feb;118(2):544–550. [PubMed] [Google Scholar]

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