Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1979 May;37(1):187–194.

Natural cytotoxic reactivity of human lymphocyte subpopulations.

M R Potter, M Moore
PMCID: PMC1457286  PMID: 288725

Abstract

The spontaneous cytotoxicity of human peripheral blood lymphocyte preparations from normal donors for K562 target cells was examined. Effector cells were separated into SRBC rosette forming cell (RFC) and non-rosette forming cell (non-RFC) fractions using optimal and suboptimal rosetting procedures. RFC and non-RFC fractions both had high cytotoxic activity irrespective of the rosetting procedure. Owing to the larger size of the RFC fraction, it contained a higher proportion of the total activity in the preparation. Nylon fibre column adherent and non-adherent fractions also both produced cytotoxicity. Nylon fibre non-adherent cells separated by SRBC separation gave a RFC fraction with low activity and a non-RFC fraction with high activity. Separation of nylon fibre adherent cells gave RFC and non-RFC fractions with high cytotoxic activity. Therefore cytotoxic cells did not form a discrete subpopulation and either occur in several lymphocyte subsets or show a variable capacity to form SRBC rosettes and adhere to nylon fibre.

Full text

PDF
191

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakács T., Gergely P., Cornain S., Klein E. Characterization of human lymphocyte subpopulations for cytotoxicity against tumor-derived monolayer cultures. Int J Cancer. 1977 Apr 15;19(4):441–449. doi: 10.1002/ijc.2910190402. [DOI] [PubMed] [Google Scholar]
  2. Ferrarini M., Moretta L., Abrile R., Durante M. L. Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cells. Eur J Immunol. 1975 Jan;5(1):70–72. doi: 10.1002/eji.1830050115. [DOI] [PubMed] [Google Scholar]
  3. Gupta S., Good R. A. Subpopulations of human T lymphocytes. I. Studies in immunodeficient patients. Clin Exp Immunol. 1977 Nov;30(2):222–228. [PMC free article] [PubMed] [Google Scholar]
  4. Jondal M., Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int J Cancer. 1975 Apr 15;15(4):596–605. doi: 10.1002/ijc.2910150409. [DOI] [PubMed] [Google Scholar]
  5. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  6. Kay H. D., Bonnard G. D., West W. H., Herberman R. B. A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1977 Jun;118(6):2058–2066. [PubMed] [Google Scholar]
  7. Kay H. D., Sinkovics J. G. Letter: Cytotoxic lymphocytes from normal donors. Lancet. 1974 Aug 3;2(7875):296–297. [PubMed] [Google Scholar]
  8. Kiuchi M., Takasugi M. The nonselective cytotoxic cell (N cell). J Natl Cancer Inst. 1976 Mar;56(3):575–582. doi: 10.1093/jnci/56.3.575. [DOI] [PubMed] [Google Scholar]
  9. Moretta L., Ferrarini M., Mingari M. C., Moretta A., Webb S. R. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976 Dec;117(6):2171–2174. [PubMed] [Google Scholar]
  10. Nelson D. L., Bundy B. M., Strober W. Spontaneous cell-mediated cytotoxicity by human peripheral blood lymphocytes in vitro. J Immunol. 1977 Oct;119(4):1401–1405. [PubMed] [Google Scholar]
  11. Oldham R. K., Djeu J. Y., Cannon G. B., Siwarski D., Herberman R. B. Cellular microcytotoxicity in human tumor systems: analysis of results. J Natl Cancer Inst. 1975 Dec;55(6):1305–1318. doi: 10.1093/jnci/55.6.1305. [DOI] [PubMed] [Google Scholar]
  12. Pape G. R., Troye M., Perlmann P. Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. II. Cytotoxicity to allogeneic or autochthonous tumor cells in tissue culture. J Immunol. 1977 Jun;118(6):1925–1930. [PubMed] [Google Scholar]
  13. Pellegrino M. A., Ferrone S., Dierich M. P., Reisfeld R. A. Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydryl compound 2-amino ethylisothiouronium bromide. Clin Immunol Immunopathol. 1975 Jan;3(3):324–333. doi: 10.1016/0090-1229(75)90019-7. [DOI] [PubMed] [Google Scholar]
  14. Peter H. H., Pavie-Fischer J., Fridman W. H., Aubert C., Cesarini J. P., Roubin R., Kourilsky F. M. Cell-mediate cytotoxicity in vitro of human lymphocytes against a tissue culture melanoma cell line (igr3). J Immunol. 1975 Aug;115(2):539–548. [PubMed] [Google Scholar]
  15. Potter M. R., Moore M. Organ distribution of natural cytotoxicity in the rat. Clin Exp Immunol. 1978 Oct;34(1):78–86. [PMC free article] [PubMed] [Google Scholar]
  16. Potter M. R., Moore M. PHA stimulation of separated human lymphocyte populations. Clin Exp Immunol. 1975 Sep;21(3):456–467. [PMC free article] [PubMed] [Google Scholar]
  17. Potter M. R., Moore M. The effect of adherent and phagocytic cells on human lymphocyte PHA responsiveness. Clin Exp Immunol. 1977 Jan;27(1):159–164. [PMC free article] [PubMed] [Google Scholar]
  18. Pross H. F., Jondal M. Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes. Clin Exp Immunol. 1975 Aug;21(2):226–235. [PMC free article] [PubMed] [Google Scholar]
  19. Rosenberg E. B., McCoy J. L., Green S. S., Donnelly F. C., Siwarski D. F., Levine P. H., Herberman R. B. Destruction of human lymphoid tissue-culture cell lines by human peripheral lymphocytes in 51Cr-release cellular cytotoxicity assays. J Natl Cancer Inst. 1974 Feb;52(2):345–352. doi: 10.1093/jnci/52.2.345. [DOI] [PubMed] [Google Scholar]
  20. Saksela E., Häyry P., Andersson L. C. Identification of the effector cells in human blood displaying spontaneous cytotoxicity to chicken erythrocytes. Eur J Immunol. 1977 Nov;7(11):761–768. doi: 10.1002/eji.1830071104. [DOI] [PubMed] [Google Scholar]
  21. Takasugi M., Mickey M. R., Terasaki P. I. Reactivity of lymphocytes from normal persons on cultured tumor cells. Cancer Res. 1973 Nov;33(11):2898–2902. [PubMed] [Google Scholar]
  22. Vessella R. L., Gormus B. J., Lange P. H., Kaplan M. E. Heterogeneity among human lymphocyte effector cells mediating spontaneous lymphocyte-mediated cytotoxicity. Int J Cancer. 1978 May 15;21(5):594–603. doi: 10.1002/ijc.2910210509. [DOI] [PubMed] [Google Scholar]
  23. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]
  24. West W. H., Cannon G. B., Kay H. D., Bonnard G. D., Herberman R. B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J Immunol. 1977 Jan;118(1):355–361. [PubMed] [Google Scholar]
  25. West W. H., Payne S. M., Weese J. L., Herberman R. B. Human T lymphocyte subpopulations: correlation between E-rosette-forming affinity and expression of the Fc receptor. J Immunol. 1977 Aug;119(2):548–554. [PubMed] [Google Scholar]
  26. West W. H., Sienknecht C. W., Townes A. S., Herberman R. B. Performance of a rosette assay between lymphocytes and sheep erythrocytes at elevated temperatures to study patients with cancer and other diseases. Clin Immunol Immunopathol. 1976 Jan;5(1):60–66. doi: 10.1016/0090-1229(76)90149-5. [DOI] [PubMed] [Google Scholar]
  27. Wybran J., Fudenberg H. H. Thymus-derived rosette-forming cells in various human disease states: cancer, lymphoma, bacterial and viral infections, and other diseases. J Clin Invest. 1973 May;52(5):1026–1032. doi: 10.1172/JCI107267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. de Vries J. E., Cornain S., Rumke P. Cytotoxity of non-T versus T-lymphocytes from melanoma patients and healthy donors on short- and long-term cultured melanoma cells,. Int J Cancer. 1974 Oct 15;14(4):427–434. doi: 10.1002/ijc.2910140402. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES