Skip to main content
. 2001 Jan 2;98(1):235–240. doi: 10.1073/pnas.98.1.235

Figure 1.

Figure 1

Ability of islets to secrete insulin after treatment with proinflammatory cytokines. Isolated islets from the NOD.scid, ALS/Lt, and ALR/Lt strains were incubated for 15 h in 5.5 mmol glucose DMEM (open bars) or 5.5 mmol glucose DMEM containing 5 units/ml IL-1β, 100 units/ml TNFα, and 100 units/ml IFN-γ (hatched bars) or 10 units/ml IL-1β, 500 units/ml TNFα, and 500 units/ml IFNγ (solid bars). Islets were washed free of the cytokine incubation media and assayed for GSIS in 22 vs. 2.8 mmol glucose DMEM. Data represent the mean difference between basal and GSIS ± SEM. Results are calculated from two independent experiments performed in triplicate.