Figure 3.

Role of autologous CD8⁺ T cells in suppression of viral replication in infected individuals receiving HAART. Highly enriched resting CD4⁺ T cells and CD8⁺ T cells from chronically infected patients who were receiving HAART (A) and those who initiated HAART during primary infection (B) were stimulated with irradiated PBMCs from seronegative donors and anti-CD3 antibodies in four different culture conditions as described in Materials and Methods. The cultures were maintained for 12 days with supernatants being removed every 3 days and replaced with fresh complete medium and anti-β-chemokine antibodies where indicated. Supernatants were assayed for p24 on days 3, 6, 9, and 12. Results of p24 ELISA on day 12 are shown. p24 values below 10pg/ml were plotted under the dotted line.