Figure 3.

Quantification of urinary porphyrins in wt and URO-D^+/− mice 3 weeks after treatment with ALA or ALA plus iron. Animals were given drinking water containing ALA (2 mg/ml), and some received an i.p. injection of iron-dextran (Fe) containing 10 mg of iron. Urinary porphyrins were separated and quantified by HPLC. Each group represents the mean value from three animals. Within a group, neither total urinary porphyrin excretion nor the proportion of uroporphyrin or partially decarboxylated porphyrins varied by more than 10%. 8COOH, uroporphyrin; 7COOH, heptacarboxyl porphyrin; 6COOH, hexacarboxyl porphyrin; 5COOH, pentacarboxyl porphyrin; 4COOH, coproporphyrin.